Characterization of sludges — Detection and enumeration of Salmonella spp. in sludges, soils, soil improvers, growing media and biowastes — Part 2: Liquid enrichment method in selenite-cystine medium followed by Rapport-Vassiliadis for semi-quantitative Most Probable Number (MPN) determination
ADOPTED_FROM:CEN/TR 15215-2:2006
This part of the CEN Technical Report method describes a method to detect and semi-quantitatively determine Salmonellae in sludges, soils, soil improvers, growing media and biowastes in accordance with the requirements of the European Sewage Sludge Regulation Revision of Directive 86/278/EEC (3rd Draft, CEN/TC 308 - doc525).
The fully defined scope will be determined after the proposed validation trials have been agreed and carried out. The method has a limit of detection of approximately 1cfu/g wet weight sample.
NOTE The objective is to cover untreated and treated sludges, soils, soil improvers, growing media, biowastes and associated materials.